Journal article
Yuye Yang, Han Yang, Zihan Yi, Lijuan Yang, Yueli Ni, Meiling Zhou, G. Xiong, Yannick Luther Agbana, Lei Zhao, Zhe Yang, Qiao Zhang, Y. Kuang, Yuechun Zhu
2020
2020
Semantic Scholar
DOI
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APA
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Yang, Y., Yang, H., Yi, Z., Yang, L., Ni, Y., Zhou, M., … Zhu, Y. (2020). LncRNA BANCR Facilitates Melanoma Cells Growth, Migration and Invasion by Inhibiting miR-206 and miR-571 to Induce G6PD Expression.
Chicago/Turabian
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Yang, Yuye, Han Yang, Zihan Yi, Lijuan Yang, Yueli Ni, Meiling Zhou, G. Xiong, et al. “LncRNA BANCR Facilitates Melanoma Cells Growth, Migration and Invasion by Inhibiting MiR-206 and MiR-571 to Induce G6PD Expression” (2020).
MLA
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Yang, Yuye, et al. LncRNA BANCR Facilitates Melanoma Cells Growth, Migration and Invasion by Inhibiting MiR-206 and MiR-571 to Induce G6PD Expression. 2020.
BibTeX Click to copy
@article{yuye2020a,
title = {LncRNA BANCR Facilitates Melanoma Cells Growth, Migration and Invasion by Inhibiting miR-206 and miR-571 to Induce G6PD Expression},
year = {2020},
author = {Yang, Yuye and Yang, Han and Yi, Zihan and Yang, Lijuan and Ni, Yueli and Zhou, Meiling and Xiong, G. and Agbana, Yannick Luther and Zhao, Lei and Yang, Zhe and Zhang, Qiao and Kuang, Y. and Zhu, Yuechun}
}
Abstract
Background Here, we evaluated the roles of LncRNA BANCR in facilitating melanoma cells growth, migration, invasion. Moreover, we examined the molecular mechanisms of BANCR in promoting melanoma development.Methods Here, we measured BANCR expression by quantitative PCR. By transwell assay, we detected the cancer cell migration and invasion. The melanoma growth and migration induced by BANCR was detected by colony formation assay and in xenograft nude mice model. By Western blotting and luciferase assay, we evaluated the molecular mechanism of BANCR in inhibiting miR-206 and miR-571 to induce G6PD expression. Results We found BANCR was overexpressed in melanoma cells and tissues. In addition, we showed that BANCR promoted melanoma cells growth in vitro and in vivo. Moreover, BANCR promotes cancer cell migration and invasion in vitro, and induced melanoma metastasis in nude mice model. The migration and invasion induced by BANCR in melanoma cells were involved in upregulation of matrix metalloproteinases (MMPs) MMP2 and MMP9. Mechanismly, we indicated BANCR promoted melanoma cell growth and migration by suppressing miR-206 and miR-571 expression to activate glucose metabolism pathway and induce G6PD expression. Furthermore, miR-206 and miR-571 inhibited G6PD expression by directly binding the 3’UTR of G6PD. We also showed miR-206 and miR-571 inhibited the proliferation of melanoma cells.Conclusion Our findings indicated that BANCR contributed to melanoma development, which might provide novel insights into the function of lncRNA-driven carcinogenesis in melanoma.
